Antimicrobial Synergy Testing Services
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Creative Diagnostics offers microdilution checkerboard assays to assess the interaction of two or more antimicrobial agents against specific pathogens. The first step is to prepare stock solutions of each drug and two consecutive dilutions, leading to at least double the MIC. Then a total of 50μl of Mueller-Hinton broth is dispensed into each well of the microdilution plate. The first antibiotic in the combination is diluted sequentially along the ordinate, while the second drug is diluted along the abscissa. An inoculum equivalent to 0.5 McFarland turbidity standard is prepared from each Pseudomonas aeruginosa isolate in Mueller-Hinton broth.
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thomasm
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